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CTA assay A week before the study start (day 7), rats were single-housed with ad libitum access to chow diet and one bottle of water (food and water were placed in the cage lid so that there was room for two water bottles)
Some protocols also explore combining IGF-1 optimization with pathways involving follistatin and myostatin inhibition, though this area continues to evolve in research settings
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A single injection of CJC-1295 with DAC produced dose-dependent GH increases lasting 6+ days and IGF-1 elevations lasting 9-11 days (Teichman et al., JCEM, 2006)
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